Table 1. Primers used in this study.
Primer | Sequence (5’–3’) | Description |
---|---|---|
G1 | CGCGGATCCATGGCAGTAAAAGTAGCAA | The forward primer of GapC, plus Bam HI site |
G2 | CCCAAGCTTTTTAGAAAGTTCAGCTAAG | The reverse primer of GapC, plus Hind III site |
Note: BamH I and Hind III restriction enzyme sites were introduced at the 5’ end of the primers as indicated italics.