Table 2. Anaerobic cultivable bacterial genotypes in the fermentation chambers of H. paralella larvae based on 16S rRNA gene sequence analysis.
| Clone code | location | Accession No. | Class a | Family a | Genus a | Closest known species (Accession No.) | Similarity (%) | Number of isolates | Percentage of total isolates |
|---|---|---|---|---|---|---|---|---|---|
| B39 | Wall Group (WG) | FJ533243 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [53%] |
Uncultured bacterium clone 34M2 (DQ079820) | 99 | 69 | 33.17% |
| B22 | FJ533241 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [87%] |
Enterobacter cloacae strain EB89 (FJ194527) | 99 | 16 | 7.69% | |
| B21 | FJ533247 |
Bacteroidia [61%] |
Porphyromonadaceae [61%] |
Petrimonas [19%] |
Uncultured bacterium (EU773762) | 91 | 11 | 5.29% | |
| B30 | FJ533242 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Citrobacter [91%] |
Citrobacter farmeri (AF025371) | 99 | 9 | 4.33% | |
| B31 | FJ533246 |
Actinobacteria [54%] |
Actinomycetales [54%] |
Micrococcineae [54%] |
Uncultured Rheinheimera sp. clone F5OHPNU07ID24Q (HQ091615) | 94 | 8 | 3.85% | |
| B15 | FJ533240 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Citrobacter [57%] |
Uncultured bacterium clone C80 (FJ356046) | 99 | 7 | 3.37% | |
| B1 | FJ533244 |
Clostridia [100%] |
Peptostreptococcaceae [100%] |
Clostridium XI [98%] | Clostridium venationis (EU089966) | 98 | 7 | 3.37% | |
| B12 | FJ533245 |
β-Proteobacteria [100%] |
unspecified Burkholderiales family [100%] |
Aquabacterium [100%] |
Uncultured bacterium clone MB14 (FJ204069) | 99 | 5 | 2.40% | |
| Q8 | Content Group (CG) | FJ533239 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [64%] |
Klebsiella pneumoniae subsp. Ozaenae (AF130982) | 98 | 15 | 7.21% |
| Q29 | FJ533237 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [46%] |
Uncultured bacterium clone 34M2 (DQ079820) | 99 | 12 | 5.77% | |
| Q2 | FJ533233 |
Bacteroidia [100%] |
Porphyromonadaceae [100%] |
Dysgonomonas [100%] |
Dysgonomonas sp. TW5-36(KR822476) | 98 | 12 | 5.77% | |
| Q13 | FJ533235 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [47%] |
Uncultured bacterium clone ZS66 (KU241008) | 99 | 9 | 4.33% | |
| Q9 | FJ533234 |
Bacteroidia [100%] |
Porphyromonadaceae [100%] |
Dysgonomonas [100%] |
Uncultured bacterium clone B68(JN006180) | 98 | 9 | 4.33% | |
| Q33 | FJ533232 |
Bacteroidia [100%] |
Porphyromonadaceae [100%] |
Dysgonomonas [100%] |
Uncultured Bacteroidetes bacterium (AB522123) | 99 | 8 | 3.85% | |
| Q20 | FJ533236 |
Bacteroidia [100%] |
Porphyromonadaceae [100%] |
Dysgonomonas [100%] |
Uncultured bacterium clone B56 (JN006178) | 98 | 7 | 3.37% | |
| Q21 | FJ533238 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [78%] |
Uncultured bacterium clone 12M2 (DQ079819) | 98 | 4 | 1.92% |
a: The taxonomic affiliation of each bacterial genotype was estimated in class, family and genus levels by using the web-based Ribosomal Database Project (RDP) classifier analysis (RDP Release 11, https://rdp.cme.msu.edu/classifier/classifier.jsp). The numbers in the brackets indicated the confidence (bootstrap) values of RDP classifier analysis.