Table 3. Aerobic cultivable bacterial genotypes in the fermentation chambers of H. paralella larvae based on 16S rRNA gene sequence analysis.
| Clone code | location | Accession No. | Class a | Family a | Genus a | Closest known species (Accession No.) | Similarity (%) | Number of isolates | Percentage of total isolates |
|---|---|---|---|---|---|---|---|---|---|
| B267 | Wall Group (WG) | FJ533267 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [99%] |
Uncultured Enterobacter sp. clone LR148 (HM597909) | 99 | 63 | 22.26% |
| B236 | FJ533265 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [59%] |
Uncultured bacterium clone PB2_aai21d08 (EU777815) | 98 | 14 | 4.95% | |
| B221 | FJ533262 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [99%] |
Trabulsiella [66%] |
Uncultured bacterium clone PCC-4 (EF608526) | 95 | 12 | 4.24% | |
| B201 | FJ533259 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [89%] |
Uncultured bacterium clone RDX 15 (EU907879) | 99 | 10 | 3.53% | |
| B272 | FJ533268 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [69%] |
Enterobacter sp. Y4 (DQ821728) | 98 | 7 | 2.47% | |
| B206 | FJ533260 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [92%] |
Enterobacter ludwigii strain YS2 (KY887767) | 99 | 6 | 2.12% | |
| B234 | FJ533263 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [50%] |
Uncultured bacterium clone EHB-PS1001 (KU978368) | 99 | 5 | 1.77% | |
| B210 | FJ533261 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [64%] |
Uncultured bacterium clone EHB-PS0427 (KU978324) | 99 | 5 | 1.77% | |
| B277 | FJ533269 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Citrobacter [91%] |
Citrobacter sp. KSL 4–091 (FJ481101) | 99 | 4 | 1.41% | |
| B247 | FJ533266 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Citrobacter [93%] |
Grimontella senegalensis (AY217653) | 99 | 2 | 0.71% | |
| B235 | FJ533264 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [24%] |
Uncultured Enterobacteriaceae bacterium clone SKF016 (JF733260) | 96 | 2 | 0.71% | |
| Q151 | Content Group (CG) | FJ533258 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [75%] |
Enterobacter cancerogenus (FM210030) | 99 | 97 | 34.28% |
| Q110 | FJ533248 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [95%] |
Uncultured bacterium clone SC24 (JF964694) | 98 | 11 | 3.89% | |
| Q164 | FJ533256 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [83%] |
Uncultured Klebsiella sp. clone LSSR193 (HM597960) | 99 | 9 | 3.18% | |
| Q159 | FJ533257 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [93%] |
Enterobacter cloacae strain XJU-PA-7 (EU733519) | 99 | 8 | 2.83% | |
| Q175 | FJ533254 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [81%] |
Klebsiella sp. Gc-7-c (FJ159440) | 99 | 8 | 2.83% | |
| Q147 | FJ533251 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [74%] |
Uncultured bacterium clone A2_806 (KR304480) | 98 | 7 | 2.47% | |
| Q189 | FJ533253 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Enterobacter [48%] |
Uncultured bacterium clone NYSYF111 (EU879477) | 98 | 4 | 1.41% | |
| Q135 | FJ533249 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [90%] |
Cedecea [27%] |
Uncultured bacterium clone JH-YT47 (EF033238) | 98 | 3 | 1.06% | |
| Q136 | FJ533250 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Pseudocitrobacter [81%] |
Uncultured Enterobacteriaceae bacterium clone SKF016 (JF733260) | 95 | 3 | 1.06% | |
| Q172 | FJ533255 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [94%] |
Uncultured bacterium (FM865635) | 99 | 2 | 0.71% | |
| Q186 | FJ533252 |
γ-Proteobacteria [100%] |
Enterobacteriaceae [100%] |
Klebsiella [97%] |
Klebsiella pneumoniae strain FIUMS1 (FJ436718) | 99 | 1 | 0.35% |
a: The taxonomic affiliation of each bacterial genotype was estimated in class, family and genus levels by using the web-based RDP classifier analysis (RDP Release 11, https://rdp.cme.msu.edu/classifier/classifier.jsp). The numbers in the brackets indicated the confidence (bootstrap) values of RDP classifier analysis.