Table 1. Function of genes and primer sequences used in the transcriptional response study.
Function | Gene | Forward primer (5’—3') | Reverse primer (5’—3') | Reference |
---|---|---|---|---|
Chaperone proteins | dnaK | TCAACCGTGTCACCCAAGTA | TCCTTCAGTTGTGGCATTCA | [31] |
groEL | ACCGGATTGAAGATGCTTTG | AACCAGCATTTTCAGCGATT | [31] | |
Proteases | clpB | AGTTACCGGCGTCCATACTG | GACTCAAAGCCGTCTCAAG | [48] |
clpC | ATCCTTTCCTCGCGAATTTT | TGGCGTTCCTTCAGTCTTCT | [49] | |
Small heat shock proteins | hsp1 | AGGTTGATGTCCCTGGTATTG | TTAAGACACCGTCAGCTTGG | [48] |
hsp2 | TTACCTTCGCTATCCCGCAAC | CGGTGAAGTATGCTGACGAA | [50] | |
hsp3 | ATCCGCAGCTGCCTTCTTT | CGCGAGTGAACGTCAAACTG | [31] | |
Cold shock proteins | cspP | TACTGGTGAAGATGGCAACG | GAACAACGTTAGCAGCTTGTGG | [44] |
cspL | GTGAAGACGGTACCGATGTCTT | GTGGTTGAACGTTCGTTGCT | [44] | |
Stress factors | ftsH | GCAGCTACCTTCGAAGAATCCA | GGGAAACTTGGTTCAGCAACA | [51] |
ctsR | AATTTGGTCGATGATGCTGATG | TAAGTCCCGGTCCGTTAATCC | [51] | |
Small redox protein | trxBI | ATGGCAAAGAGTTACGACG | CCCACCATAGATTCCGCGAT | F: [52] R: this study |
Adhesion protein | efTU | CCACGTAATAACGCACCAAC | TTCTGGTCGTATCGATCGTG | [29] |
Plantaricin synthesis | plnE | GTTTTAATCGGGGCGGTTAT | ATACCACGAATGCCTGCAAC | [32] |
plnJ | TAAGTTGAACGGGGTTGTTG | TAACGACGGATTGCTCTGC | [32] | |
D-lactate dehydrogenase | ldhD | ACGCCCAAGCTGATGTTATATC | AGTGTCCCACGAGCAAAGTT | [53] |