Table 5. Summary of mGlu₇ PAM VS and Resulting Hits.

method	PSa compds tested	PS activeb	PS hit rate	conf compds testedc	pEC50 > 4.52 ag or PAMd	active in autofluorescence	no. of confirmed actives	final confirmed hit rate
single target approach: fingerprint analogues of only mGlu7 actives	202	27	13%	25	17	12	5	2.5%
multitarget approach: select molecules based on likelihood to be mGlu7 active from PCM	394	42	11%	41	18	1	17	4.3%

^aPS compds tested refers to compounds tested in the primary screen.

^b>50% effect at 3 or 10 μM in either agonist or PAM assay.

^cconf compds tested refers to number of compounds tested in confirmation assays.

^dAg refers to agonist.