Table 3.
Effects of the pretreatment with hydroalcoholic fraction of C. spinosa and Quercetin on the serum activities of AST, ALT, ALP and sleeping time in t-BHP-induced hepatotoxicity.
| Groups | ALT (U/l) | AST (U/l) | ALP (U/l) | Sleeping time (min) |
|---|---|---|---|---|
| 1 – Normal saline (control) | 77.35 ± 5.073 | 119.1 ± 7.898 | 303.9 ± 7.426 | 15.44 ± 1.613 |
| 2 – Extract (400 mg/kg) | 90.94 ± 3.54b | 113.3 ± 6.617b | 301.4 ± 11.17b | 18.29 ± 1.604b |
| 3 – t-BHP (50 mg/kg) | 357.33 ± 25.94a | 491.7 ± 26.53a | 430.9 ± 15.25a | 43.53 ± 2.977a |
| 4 – Extract (100 mg/kg) + t-BHP | 241.41 ± 17.19a,b | 308.5 ± 22.23a,b | 405.2 ± 6.501a | 38.85 ± 2.667a |
| 5 – Extract (200 mg/kg) + t-BHP | 160.21 ± 17.72a,b | 311.4 ± 16.43a,b | 378.8 ± 13.67a | 30.97 ± 1.771a,b |
| 6 – Extract (200 mg/kg) + t-BHP | 129.80 ± 15.93b | 236.8 ± 16.49a,b | 340.7 ± 21.67b | 24.60 ± 2.011b |
| 7 – Quercetin (200 mg/kg) + t-BHP | 192.42 ± 14.90a,b | 219.4 ± 12.70a,b | 326.7 ± 21.44b | 26.80 ± 2.092a,b |
Animals in group 1 received normal saline solution, while group 2 received C. spinosa extract (400 mg/kg, po) for 5 days, group 3 received t-BHP (0.18 mM/kg, ip) on the fifth day. The mice in groups 4, 5 and 6 were pretreated with C. spinosa extract (100, 200 and 400 mg/kg, p.o, respectively) once daily for five consecutive days. Group 7 were pretreated with Quercetin (1.5 mM/kg, p.o), once daily for five consecutive days. One hour after the final treatment, the mice were treated with t-BHP (0.18 mM/kg, ip). Hepatotoxicity was determined 24 h later by quantifying the sleeping time and serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) as well as alkaline phosphatase (ALP).
Each value represents the mean ± SEM for eight mice.
Significantly different from the control (p < 0.05).
Significantly different from t-BHP group (p < 0.05).