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. 2017 Dec 26;15:347–362. doi: 10.1016/j.redox.2017.12.012

Fig. 1.

Fig. 1

HE-derived nonspecific oxidation and O2•–-specific hydroxylation products. The fluorescence spectra of the nonspecific oxidation product E+ (10 µM, right axis) and O2•–-dependent hydroxylation product 2-OH-E+ (10 µM, left axis) in the presence of DNA show a significant overlap. (Obtained from Ref. [15]. Reprinted from H. Zhao, J. Joseph, H.M. Fales, E.A. Sokoloski, R.L. Levine, J. Vasquez-Vivar, B. Kalyanaraman, Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence, Proceedings of the National Academy of Sciences of the United States of America 102(16) (2005) 5727–5732, Copyright 2005 National Academy of Sciences.).