Table 1.
Primary Antibodies Used in This Study
| Antibody | Source | Dilution | Antigen retrieval |
|---|---|---|---|
| Mouse anti-endotubin (5F11) | Wilson Lab (University of Arizona, Tucson, AZ) | IF 1:3 Immunoblot 1:5 |
None |
| Rabbit anti-Rab 11a | Invitrogen (cat. 71-5300) | ICC 1:100 | Citrate, pH 6.0 |
| Rabbit anti-Rab 14 | Sigma (cat. R0656) | ICC 1:500 | Tris/EDTA, pH 9.0 |
| Rabbit anti–claudin 1 | Invitrogen (cat. 51-9000) | ICC 1:50 Immunoblot 1:200 |
Tris/EDTA, pH 9.0 |
| Rabbit anti-claudin 2 | Invitrogen (cat. 51-6100) | IF 1:200 Immunoblot 1:500 |
Tris/EDTA, pH 9.0 |
| Rabbit anti-Lamp1 | Abcam (Cambridge, MA) (cat. ab24170) | IF 1:200 | Citrate, pH 6.0 |
| Rabbit anti-NHE3 | Kiela Lab (University of Arizona, Tucson, AZ) | IF 1:1000 | Tris/EDTA, pH 9.0 |
| Rabbit anti-EEA1 | Abcam (cat. ab2900) | ICC 1:500 | Citrate pH, 6.0 |
ICC, immunocytochemistry; IF, immunofluoresence.