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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: Mol Cell. 2017 Dec 21;69(1):62–74.e4. doi: 10.1016/j.molcel.2017.11.031

Figure 1. UGUA is not an essential cis-element, but an enhancer for mRNA 3′ rocessing.

Figure 1

(A) Enrichment score (log2(frequency in PAS/frequency in random sequence)) for UGUA and A(A/U)UAAA. (B) RNA substrates used in this study: L3 and p14/Robld3 PAS. (C) Compare L3 wild type (WT), Δ1, Δ1-2, Δ1-3UGUA mutant PASs using in vitro mRNA 3′ processing assays. Top panel: coupled cleavage/polyadenylation assay. Bottom panel: cleavage assay. Quantification results are shown below of the gel: % processed= (5′cleavage product)/(pre-mRNA). (D) Compare p14 WT, 1x and 2xUGUA mutant PASs using in vitro mRNA 3′ processing assays. Results are shown similar to (C).