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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: Mol Cell. 2017 Dec 21;69(1):62–74.e4. doi: 10.1016/j.molcel.2017.11.031

Figure 3. Enhancer-bound CFIm promotes the assembly of mRNA 3′ processing complex.

Figure 3

(A) Gel mobility shift assays with L3 or p14-derived PASs. P: mRNA 3′ processing complex; H: heterogenous complex. (B) A diagram showing the RNA affinity purification procedure. MBP-MS2: a fusion protein between maltose binding protein and MS2. (C) The complexes assembled on the 3MS2-tagged L3 or p14-derived PASs were purified and analyzed by western blotting. The red arrows mark the CFIm subunits. (D) mRNA 3′ processing complex assembly on L3-derived PASs as shown in Fig. 2(A). (E) The mRNA 3′ processing complexes assembled on the 3MS2-tagged L3 derivatives as shown in Fig. 2(A) were purified and analyzed by western blotting. (F) Quantification of western blot signals in (E) using ImageJ.