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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: Mol Cell. 2017 Dec 21;69(1):62–74.e4. doi: 10.1016/j.molcel.2017.11.031

Figure 4. The RS-like domain of CFIm68/59 is necessary and sufficient for activating mRNA 3′ processing.

Figure 4

(A) A diagram of the tethering assay. RRM: RNA recognition motif; PRR: proline-rich region; RS: arginine-serine repeat region. (B–E) Tethering assay results obtained by co-expressing the L3-2xBoxB reporter and the proteins as labeled. The CFIm25 mutant L218R was labeled vertically. The results were plotted as mean ± s.e.m (n=3). PAS activities for tagged and untagged proteins were compared. L218R was compared to the wild type CFIm25. *** indicates that the p-values<0.001 (t-test). All samples were compared with the vector and *** indicates that the p-values<0.001 (t-test).