REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CPSF160	Bethyl	A301-580A; RRID:AB_1078859
CPSF100	Bethyl	A301-581A; RRID:AB_1078861
Fip1	Bethyl	A301-091A; RRID:AB_2084528
CstF64	Bethyl	A301-092A; RRID:AB_873014
CFIm68	Bethyl	A301-358A; RRID:AB_937785
CFIm59	Bethyl	A301-360A; RRID:AB_937864
CFIm25	Santa Cruz	sc-81109; RRID:AB_2153989
hnRNP A1	Santa Cruz	sc-56700; RRID:AB_629651
Chemicals, Peptides, and Recombinant Proteins
DMEM (high glucose)	Thermo Fisher	11995-073
Dynabeads Protein A	Thermo Fisher	10002D
Dynabeads Streptavidin	Thermo Fisher	658.01D
Glutathione Sepharose High Performance beads	GE Healthcare Life Sciences	17527901
Shrimp Alkaline Phosphatase (SAP)	Thermo Fisher	EF0511
6xHis-CFIm25/68	This study	N/A
6xHis-CFIm25/59	This study	N/A
GST-CFIm59 RS	This study	N/A
GST-CFIm68 RS	This study	N/A
Fip1-RD peptide	GenScript	Custom synthesis: SC1208/U2711BI160_1
Fip1-RA peptide	GenScript	Custom synthesis: SC1208/U2711BI160_4
Critical Commercial Assays
TnT® Quick Coupled Transcription/Translation System Kit	Promega	L1170
Dual-Luciferase Reporter Assay Kit	Promega	E1910
FuGENE® HD Transfection Reagent	Promega	E2311
Phos-tag™	Wako	304-93521
Deposited Data
PAS-seq	This study	GSE101871
Raw experimental data	This study	http://dx.doi.org/10.17632/r23kcs7s8n.1
Experimental Models: Cell Lines
Human: CFIm68 KO cells	Dr. Alan Engelman	Sowd et al., 2016
Human: CFIm59 KO cells	This study	N/A
Sf9 Insect cells	This study	N/A
Oligonucleotides
Primers for cloning and qPCR	This study	See Table S1
Recombinant DNA
Plasmids for transfections and in vitro assays	This study	See Table S2
Software and Algorithms
deepTools	Ramirez et al., 2016	http://deeptools.readthedocs.io/en/latest/
diffSpliceDGE and topSpliceDGE	Robinson et al., 2010	http://bioconductor.org
BEDTools	Quinlan and Hall, 2010	http://bedtools.readthedocs.io/en/latest/
SAMtools	Li et al., 2009	http://samtools.sourceforge.net/