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. Author manuscript; available in PMC: 2019 Jan 4.
Published in final edited form as: Cell Stem Cell. 2017 Nov 30;22(1):104–118.e6. doi: 10.1016/j.stem.2017.10.005

Figure 3. A HIF1α/STAT3 Co-Activator Complex Induces Expression of Vasorin in GSCs.

Figure 3

(A) Cartoon of Vasorin promoter with 3 putative hypoxia response elements (HRE) and 1 STAT3 binding site.

(B–D) Immunblot (IB) of Vasorin in GSCs transduced with two different shRNA sequences targeting HIF1α (B), HIF2α (C) or STAT3 (D). GSCs were cultured in hypoxia (1% O2) for 48 hrs.

(E) qRT-PCR of HIF1α and Vasorin in GSCs transduced with shNT or shHIF1α and cultured in hypoxia for 48 hrs.

(F) qRT-PCR of STAT3 and Vasorin in GSCs transduced with shNT or shSTAT3 and cultured in hypoxia for 48 hrs.

(G–H) Chromatin IP (ChIP) analyses on the promoter of Vasorin. Assays were performed with the indicated antibodies and immunoprecipitates were subjected to qPCR analyses using primers flanking the HRE sites (G) or STAT3 binding motif (H). GSCs were cultured in hypoxia for 48 hrs.

(I) ChIP of HIF1α binding to the Vasorin promoter in GSCs cultured under hypoxia after STAT3 knockdown.

*p < 0.05; **p < 0.01, *** p < 0.001. Data are represented as mean ± SD. See also Figure S3.