Table 4.

Summary of mutation positive cases identified by tagged amplicon sequencing

Sample	Histology	Mol. group	Mutation	Depth	Mutation allele frequency		Average	Additional mutations
					Duplicate 1	Duplicate 2
GNT03	GG	1	BRAF-V600E	207	8%	N/A	8%	N/A
GNT21	GG	1	BRAF-V600E	226	7%	8%	8%	N/A
GNT29	GG	1	BRAF-V600E	163	8%	N/A	8%	N/A
GNT35	GG	1	BRAF-V600E	1455	28%	32%	30%	N/A
GNT40	GNT NOS	1	BRAF-V600E	726	23%	25%	24%	N/A
GNT48	GG	1	BRAF-V600E	71	1%	1%	1%	N/A
GNT51	GG	1	BRAF-V600E	2948	23%	25%	24%	N/A
GNT52	GNT NOS	1	BRAF-V600E	79	7%	6%	7%	CTNNB1-G34E
GNT16	GG	1	BRAF-V600E	6370	17%	20%	19%	N/A
GNT01	GG	1	BRAF-V600E	6277	26%	23%	25%	N/A
GNT05	GG	1	BRAF-V600E	4400	18%	20%	19%	N/A
GNT17	GG	1	BRAF-V600E	2197	17%	18%	18%	N/A
GNT18	GG	1	H3F3A-K27M	3047	36%	24%	30%	N/A
GNT94	GG	N/A	BRAF-V600E	707	11%	13%	12%	N/A
GNT60	GG	N/A	BRAF-V600E	282	9%	8%	9%	N/A
GNT69	GG	N/A	BRAF-V600E	852	14%	15%	15%	N/A
GNT76	GG	N/A	BRAF-V600E	343	18%	11%	15%	N/A
GNT27	GG	N/A	BRAF-V600E	1403	19%	20%	20%	N/A
GNT95	GNT NOS	N/A	BRAF-V600E	1149	13%	15%	14%	N/A
GNT81	GNT NOS	N/A	BRAF-V600E	1482	14%	15%	15%	N/A
GNT39	GNTNOS	N/A	BRAF-V600E	5016	33%	N/A	33%	N/A
GNT96	GG	N/A	BRAF-V600E	2519	25%	25%	25%	N/A
GNT87	GNT NOS	N/A	BRAF-V600E	1097	11%	12%	12%	N/A
GNT89	GG	N/A	BRAF-V600E	1299	16%	16%	16%	N/A
GNT50	GNT NOS	N/A	BRAF-V600E	3185	30%	28%	29%	N/A
GNT54	GG	N/A	BRAF-V600E	558	17%	N/A	17%	N/A
GNT92	GNT NOS	N/A	BRAF-V600E	1694	18%	19%	19%	N/A
GNT44	GNT NOS	N/A	BRAF-V600E	196	34%	8%	21%	N/A