Fig. 1.

Photomicrograph of rat testis sections: A) control-sham group, seminiferous tubules with no histological changes in germinal cell proportion and no edema in the interstitial tissue are present. The spermatogenesis and spermiogenesis processes are normal and seminiferous tubules with a high proportion of spermatozoa are observed. (H & E, 400×), B) The CPF group on day 15, edema in interstitial connective tissue and irregular arrangement and detachment of spermatogenic cells are observed in some areas. (H & E, 400×), C) The CPF group on day 45, showing severe germ cell loss and vacuolization, sloughing of germ cells into the tubular lumen and impaired spermatogenesis; seminiferous tubules with negative tubular differentiation and repopulation indices as well as severe edema and infiltration of immune mononuclear cells (1) in interstitial connective tissue can be seen. (H & E, 400×) and D) The CPF group on day 30, exhibiting interstitial connective tissue edema, partially sloughing of germ cells into the tubular lumen and negative repopulation index. Spermatogonium type B (1), Spermatogonium type A (2). Iron-weigert staining technique (400×).