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Rat spermatozoa stained with A) Eosin-nigrosin, viable sperm (1) remained colorless while non-viable sperm (2) stained red, B) Aniline-blue, mature sperm (1) with discolored protamine and immature sperm (2) characterized by nuclear histone proteins stained dark-blue and C) Acridine orange, green spermatozoa with double-stranded DNA (1) and yellow spermatozoa with single-stranded DNA (2). (1000×).
