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. 2017 Aug 5;135(1):131–148. doi: 10.1007/s00401-017-1756-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Hspb8 aggregates and low autophagy power in the sciatic nerve of Hspb8^K141N/K141N mice. a, b Hspb8 immunohistochemistry in 18-month-old WT (a) and Hspb8^K141N/K141N (b) mice showing that only the Hspb8^K141N/K141N mice develop aggregates (b). c Western blot showing Hspb8, Bag3 and p62 and in sciatic nerve lysates of 2-and 12-month-old Hspb8^K141N/K141N and WT mice. GAPDH is used as a loading control. d Quantification of the Hspb8, Bag3, and p62 bands normalized on GAPDH. *p < 0.05. Values are expressed as mean ± SD, N = 3