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Sensitivity evaluation of LAMP and PCR assays using serial dilutions of Leishmania DNA in infected sand flies. (A) Single round-PCR by targeting ITS-rDNA gene (amplified fragment; 480 bp) (B) Agarose gel electrophoresis of LAMP products electrophoresis detection. (C) LAMP, visual detection by fluorescence. M=100 bp DNA ladder marker; +Ve: Positive control; −Ve: negative control
