Fig. 3.

Notch receptor expression in primary mouse PaSCs. a Representative western blotting images showing the Notch1–4, CSL and HES1 protein expression in non-activated and activated PaSCs; densitometry analyses of the blots are also shown (groups 1 and 3 represent non-activated PaSCs; groups 2 and 4 represent activated PaSCs). b RT-qPCR results showing the Notch1–4 and HES1 mRNA expression in non-activated and activated PaSCs. c Representative double immunofluorescence staining of α-SMA (red) and Notch3 (green) in primary mouse PaSCs. Scale bars: 50 μm in (c). The data are presented as the mean ± SD, **P < 0.01 and ***P < 0.001; n = 4; Student’s t-test