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. 2018 Jan 5;19:3. doi: 10.1186/s12931-017-0708-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Flow cytometric discrimination of alveolar and exudative macrophages by surface marker expression. Representative FACS plots from an IAV-infected LM mouse at 10 dpi, which detail our 12-color flow cytometry gating strategy of single cell suspensions from BAL and enzyme-digested lung (a). Alveolar macrophages (AM) were designated as F4/80⁺ SiglecF⁺ CD11b^neg/dim, whereas exudative macrophages (EM) were designated as F4/80⁺ SiglecF^neg/dim CD11b+. Supra-physiologic GM-CSF levels during IAV infection had no effect on the absolute number of either airway (BAL-recovered) AM or EM cell numbers at 10 dpi (b)