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. 2018 Jan 5;11:4. doi: 10.1186/s13048-017-0376-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d. Isolated follicles after using collagenase NB6; b and e. Isolated follicles after using Liberase DH; c and f. Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm