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. 2018 Jan 5;11:4. doi: 10.1186/s13048-017-0376-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Quantification of leukemic cells by MFC in an ovarian suspension containing isolated follicles. Only viable nucleated cells (SYTO13⁺/7-AAD⁻), which are CD45_low were represented (purple dots). Black dots represent leukemic cells detected with a typical immunophenotype CD38⁺/CD43⁺/CD361⁺/CD117⁺ among the population of CD45_low cells. A positive event must be at the intersection of gates P1 (CD38 versus CD43), P2 (CD43 versus CD361), and P3 (CD38 versus CD117). a Positive control (leukemic cells); b MFC analysis of follicles isolated from healthy ovarian suspension after addition of 10⁶ leukemic cells, before any wash (n = 2069 events detected); c MFC analysis of isolated follicles after 3 washes (n = 7 events detected)