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. 2018 Jan 5;15:5. doi: 10.1186/s12985-017-0915-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Binase is not reducing the amount of host-cell transcripts and expressed proteins. a Real-time RT-PCR to analyze the expression levels of the housekeeping genes mRNA transcripts ATPase (ATP6V0E1) and Tubulin (TUBA4A) in binase-treated (10⁵ U/ml) and non-treated MDCK-II cells at 12 h p.i. b Western blot analysis of cellular beta-actin protein expression levels in binase treated (10⁵ U/ml) and non-treated MDCK-II cells. Statistical significance was assessed using the Student’s t-test. *P < 0.1