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. 2018 Jan 5;15:5. doi: 10.1186/s12985-017-0915-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Impact of binase on the viral expression efficiency of the H1N1pdm09 RNP complex. Effect of binase on the viral expression activity of the H1N1pdm09 RNP complex was analyzed in 293 T cells transfected with plasmids expressing the polymerase subunits (PB1, PB2, and PA) and NP of H1N1pdm09 together with vRNA-like transcript (pPol1-CAT-RT) encoding the CAT reporter gene, was analyzed for CAT activity 48 h post transfection. The cells were treated with 10⁴ and 10⁵ U/ml of binase after transfection. In the control, binase was absent. Statistical significance was investigated using the Student’s t-test. ***P < 0.001