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. 2018 Jan 1;27(1):10–22. doi: 10.1089/scd.2017.0132

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© Pavel Simara et al. 2017; Published by Mary Ann Liebert, Inc.

This article is available under the Creative Commons License CC-BY-NC (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Permission only needs to be obtained for commercial use and can be done via RightsLink.

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FIG. 2. — Detection of Ig and TCR gene recombinations in hiPSC-PB. Genomic DNA from PBMCs is provided as a positive control, while fibroblast line (HDFn) serves as a negative control. Sixty-three primers were used in seven multiplex PCR tubes. (A) Complete VH-JH rearrangement of IGH gene. Three tubes were used with valid sizes of amplicon (1) 310–360 bp, (2) 250–295 bp, and (3) 100–170 bp. (B) Complete Vβ-Jβ rearrangement of TRB gene. Two tubes were used with valid sizes (4) 240–285 bp and (5) 240–285 bp. (C) TRG gene rearrangements. Two tubes were used with valid sizes (6) 145–255 bp and (7) 80–220 bp. MwM, molecular weight marker. HDFn, human dermal neonatal fibroblast; hiPSC-PB, hiPSC derived from PBMCs; Ig, immunoglobulin; PCR, polymerase chain reaction; TCR, T-cell receptor.