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. 2018 Jan 1;27(1):10–22. doi: 10.1089/scd.2017.0132

FIG. 4.

FIG. 4.

Functional characterization of ECs derived from hiPSC-PB, hiPSC-HU, and hiPSC-HS. HUVEC and HSVEC serve as positive controls, while HDFn are provided as negative controls. (A) Tube formation assay. The number of complete rings was assessed by image analysis described in Materials and Methods section and labeled by black cross. Scale bar = 200 μm. (B) Quantification of the tube formation assay. Each column indicates mean number of complete rings counted in three independent wells of 96-well plate (±SEM). Asterisk indicates statistically significant (P < 0.05) difference in the HDFn ring count compared with the ECs ring counts, as detected using Student's t-test. (C) Chemotaxis migration assay. Only the cells on the outer side of the membrane which were attracted by VEGF (50 ng/mL) are shown. Scale bar = 100 μm. (D) Quantification of the chemotaxis migration assay. Each column indicates mean number of cells counted in three independent cell culture inserts (±SEM). For each insert, three images were manually counted. Asterisk indicates statistically significant (P < 0.05) difference in the HDFn cell count compared with the ECs cell counts, as detected using Student's t-test.