Fig. 3.

80  % FiO₂ increases neutrophil activation in P. aeruginosa-infected muscle. Rats treated with 80 % or 30 % FiO₂ were either injected with saline or infected with 5×10⁶ P. aeruginosa (PA) into biceps femoris muscle after surgery. (a) Muscle tissue lysates were collected from the different treatment groups 24 h following surgery and probed for myeloperoxidase (MPO) by Western blotting. The experiment was performed in duplicate and repeated over four trials. (b) For each sample measured, the MPO levels were normalized to GAPDH. Data are shown as mean±sem, ANOVA with the lsd post hoc test.