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. Author manuscript; available in PMC: 2019 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2017 Aug 12;461:22–31. doi: 10.1016/j.mce.2017.08.005

Figure 2. PRKD1 loss in BMMSCs has little effect on alkaline phosphatase activity in vitro.

Figure 2

Floxed BMMSCs were infected with adenovirus expressing GFP or Cre (or uninfected) as in Figure 1. The cells were then treated for 10 days with control medium (containing 2% FBS) or osteogenesis-stimulating (OS) medium containing dexamethasone, β-glycerophosphate and ascorbic acid in the presence and absence of 50 ng/mL BMP2 (added for the first 48 hours only), and alkaline phosphatase staining determined. Shown is a representative of 3 experiments.