Figure 4. Bone marrow stromal cells and bone from osteoprogenitor-specific conditional PRKD1 knock-out mice show reduced expression of PRKD1, but not PRKD2.

Floxed PRKD1 mice were crossed with a transgenic mouse model in which the doxycycline-regulated osterix promoter drives Cre expression. (A and B) Adherent bone marrow cells were isolated from floxed and conditional PRKD1 knockout femora and (A) PRKD1 and (B) PRKD2 expression measured by qRT-PCR. (C and D) RNA was also isolated from bone shaft and (C) PRKD1 and (D) PRKD2 expression monitored by qRT-PCR. Each symbol represents an individual mouse; the line denotes the mean fold expression relative to the average control value. (E) Tissue-specific Cre expression was demonstrated by measuring Cre mRNA levels in kidney, spleen, heart and relative to bone using qRT-PCR with Gapdh as the normalization control in the osteoprogenitor-specific PRKD1 KO and floxed control mice. Results represent the means ± SEM of 3–4 individual 3-month-old male mice.