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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Dec 20;495(3):2221–2226. doi: 10.1016/j.bbrc.2017.12.111

Figure 1. Lipotoxic and glucolipotoxic conditions promote lipid accumulation in INS-1 832/13 cells without affecting CD36 expression.

Figure 1

Panel A: INS-1 832/13 cells were cultured in a medium containing low glucose (2.5 mM), high glucose (20 mM) or palmitate (2.5 mM glucose plus 0.5 mM palmitate or 20 mM glucose plus 0.5 mM palmitate) for 24 hrs. Lipid accumulation was determined by Oil-Red-O (ORO) staining in fixed cells and observed under microscope.

Panel B: INS-1 832/13 cells were serum starved (12-15 hours) and then incubated in a medium containing low glucose (2.5 mM) with 3.75% BSA (Con), GLT (20 mM glucose plus 0.5 mM palmitate) for 24 hrs. CD36 expression was quantified by Western blotting and densitometry. β-actin was used as loading control. Data are expressed as mean ± SEM from four independent experiments.