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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Exp Eye Res. 2017 Oct 31;166:120–130. doi: 10.1016/j.exer.2017.10.015

Figure 1. Generation of Spata7 conditional knockout mice.

Figure 1

(A) Spata7 targeting strategy. Numbered rectangles represent Spata7 exons. (B) Genomic Southern blot of Spata7 targeted allele. Fragments of 5.8 kb and 11.4 kb are detected in a heterozygous mouse using 5’ (HindIII) and 3’ (EcoRV) probes, respectively. (C) Western blot of SPATA7 protein probed using anti-SPATA7 antibody using retinas isolated from wild-type and Spata7Δ1–5 knockout mice. Anti-β-actin antibody was used as a loading control.