Figure 4. Spata7^Flox/−; Crx-Cre mice exhibit reduced amplitudes of scotopic and photopic ERGs.

Quantitative evaluation of scotopic (dark-adapted) a-wave and b-wave amplitudes was performed at (A, B) 1 month and (D, E) 4 months of age. Photopic (light-adapted cone response) amplitudes were also obtained at (C) 1 month and (F) 4 months of age from Spata7^Flox/+; Crx-Cre control mice and Spata7^Flox/−; Crx-Cre cKO mice. Spata7^Flox/+; Crx-Cre mice had normal scotopic and photopic ERG responses, while Spata7^Flox/−; Crx-Cre cKO mice exhibited a significantly reduced response to light, with a 50% reduction in the a-wave amplitude under scotopic conditions (A,B) and a 20–30% reduction under photopic conditions (C) at 1 month of age. At 4 months, Spata7^Flox/−; Crx-Cre cKO mice showed a further reduction in the scotopic response (D, E) and a consistent reduction in the photopic response (F). Statistical analysis was performed using the Student’s t-test (NS= not significant, p>0.05; *p<0.05; **p<0.01; ***p<0.001). n = 6 mice per genotype.