Fig. 2.

Tumor characterization. Characterization of the various surgically resected tumors is shown. (A) Tumor location and putative ancestry based on shared driver mutations are indicated with a color code. (B) Tumors were assessed by immunohistochemistry and qualitative staining patterns (− negative; + slightly positive; ++ moderately positive; +++ strongly positive) are given for different immune and tumor markers. Staining for Hep Par 1 and CK7 supported the initial diagnosis of cholangiocarcinoma (L06/10) and staining of P03/13 for thyroid transcription factor-1 (TTF1) and Napsin A ruled out a primary lung adenocarcinoma. Further, counts for immune cell infiltrates in the epithelial compartment are given for each tumor and microscopic pictures (×100) of perforin staining are shown. More detailed data is provided in Supplementary Table 2.