Fig. 8.

Overexpression of CsMYB5b in wild-type tobacco. a Phenotypes of CK and CsMYB5b transgenic tobacco flowers. b Semi-quantitative RT-PCR analysis of the CsMYB5b and the housekeeping gene NtActin transcription levels in total RNA from the flowers shown in a. c DMACA staining of the extracts from transgenic tobacco flowers and controls. d The absorbance of the anthocyanins extracts and DMACA-reactive compounds at 525 and 640 nm, respectively. e The content (peak area) of glycosides extracted from different transgenic lines of overexpressing CsMYB5b tobacco flowers and CK. f The contents (μg/g, FW) of C, EC, PA-dimer and PA-trimer extracted from different transgenic lines of overexpressing CsMYB5b tobacco flowers and CK. The PA-trimer was also quantified according to the standard procyanidin B2. All data are the means of three biological replicates, and the error bars represent the standard deviation of three replicates. The asterisks indicate the significant level (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001) based on a Tukey’s honestly significant difference test. g Expression profiles of structural genes in flowers of different transgenic CsMYB5b tobacco lines