Figure 1. Effect of frozen (A) or freshly prepared (B) conditioned medium of PC12 cells on cell attachment. The cells at the indicated cell density were inoculated without (l) or with (l, n) 50% conditioned medium, and incubated for 48 hours to determine the attached cell number by MTT method. Each value represents mean±S.D. of triplicate assay. Right panels are the logarithms of the corresponding left panels.
