Figure 7. Fisetin affects the translocation of apoptotic-associated proteins in HSC3 cells. Cells were treated with 40 μM fisetin for 24 h and cells were stained by antitobodies to cytochrome c (A), apoptosis-inducing factor (AIF) (B) and endonuclease G (ENDO G) (C) and then were stained with a secondary antibody and photographed by a Leica TCS SP2 confocal laser microscopic system as described in the Materials and Methods section.