Fig. 3. Hypothalamic mRNA levels of toll-like receptor 4 (TLR4) (A), pro-opiomelanocortin (POMC) (B), and neuropeptide Y (NPY) (C) after treatment with udenafil and/or leptin for 9 days. The mRNA levels were analyzed using real-time polymerase chain reaction. A-: AIN-93G diet, H-: high-fat diet with 60% of the calories from fat, CON: control, LEP: leptin, UDE: udenafil. Data are expressed as mean±standard error of 7 mice in each experimental group. ^*p<0.05, vs. A-control. Values that do not share a common superscript letters are significantly different at p<0.05 in the control- or high-fat-fed mice.