FIG. 4.

Effects of Microfluidic Sorting on Myotube Growth. Shortly before delamination, light microscopy images were captured on Day 14 to assess the size and density of myotubes within the developing muscle monolayer. (A, B) Representative images of monolayers from unsorted cells and cell sorted into Channel 1. Scale Bar = 500 μm. (C) The average myotube diameter was indistinguishable between the two groups: 16.0 ± 1.3 μm in controls and 16.18 ± 1.4 μm in Channel 1 (p = 0.938). (D) Channel 1 cells, however, exhibited a significantly denser myotube network (p = 0.004), with 33.9 ± 3.7 tubes/mm² in comparison to 18.6 ± 3.3 myotubes/mm² in unsorted controls. * Indicates statistical difference from control.