FIG. 4.

(A) Micrographs of lymphocytes invading the retina. CD3 positivity identifies these cells as T-lymphocytes. Co-staining of CD3 and CD4 indicates Helper T-cells, and combined CD3/CD8 positivity identifies cytotoxic T-cells. Scale bar = 10μm. (B) Time course of lymphocyte counts in flat-mounted retinas. No lymphocytes were found in the control situation. There is an initial increase of CD3+ lymphocytes at 7 days after blast. As CD3+ cells decrease, the number of CD4+ and CD8+ cells increases over the course of a month. The gray shaded area denotes the 95% confidence interval per group. (C) Correlations of Cd3 to the cytokine-related genes Cxcr3 (chemokine receptor 3), Ccl4 (chemokine ligand 4), and Ifng (interferon gamma). A drastic increase in ranked correlation and Pearson's correlation coefficient after blast can be seen. Color image is available online at www.liebertpub.com/neu