Table 3.
Method validation parameters for the studied model alkaloids: linearity, precision, limits of detection, and quantification.
| Compound | Regression equation | R 2 | Linearity range (µg/mL) | LOD (ng/mL) | LOQ (ng/mL) | Intraday precision (n = 5) [%] | Interday precision (n = 5) [%] |
|---|---|---|---|---|---|---|---|
| Berberine | y = 363158032093x + 856955661 | 0.998 | 0.1–100 | 47 | 157 | 2.6 | 3.1 |
| Berbamine | y = 55985070215x + 4453198619 | 0.997 | 0.1–250 | 25 | 84 | 3.4 | 4.2 |
| Magnoflorine | y = 35358955125x + 9667117613 | 0.998 | 0.1–100 | 18 | 61 | 1.8 | 2.1 |
| Sanguinarine | y = 7879756395x + 48208329 | 0.997 | 0.1–100 | 22 | 73 | 1.2 | 1.4 |