Table 2.

Performance metrics for ctDNA NGS test (calculated based on clinical validation of 132 patient samples)

Patient type	Patient count				Sensitivity (%)	Specificity (%)	Accuracy (%)	Precision (%)
	True positive	False negative	True negative	False positive
Exon-19 mutated	34	2	96	0	94.44	100	98.48	100
Exon-21 mutated	7	2	123	0	77.78	100	98.48	100
Overall mutated	41	4	87	0	91.11	100	96.97	100

Notes: True positive: samples predicted as EGFR mutated in allele specific real time PCR and ctDNA NGS tests. False negative: samples with EGFR mutation in allele specific real time PCR but Wild type in ctDNA NGS test. True negative: samples predicted as Wild type EGFR in allele specific real time PCR and ctDNA NGS tests. False positive: samples predicted as Wild type EGFR in allele specific real time PCR test but mutated EGFR in ctDNA NGS test.

Abbreviations: ctDNA, circulating tumor DNA; NGS, next generation sequencing.