Figure EV3. Transcriptome analysis in T^{Y 88A} mutants in vivo .

1. FACS plots from the sorting of T‐mCHERRY‐positive cells from T ^WT and T ^Y88A embryos for RNA‐seq.
2. Scatter plot of FPKM values from RNA‐Seq using mesodermal cells of T ^Y88A embryos at E8.25 and E8.5 (one biological replicate for each timepoint).
3. Scatter plot of log₂ fold change values comparing T ^Y88A mutant mesodermal cells to T ^WT at E8.25 and E8.5 (log₂ fold change ≥ 1.3).
4. Venn diagrams illustrating genes that are dysregulated both at E8.25 and E8.5 in T ^Y88A mutant mesodermal cells compared to T ^WT (log₂ fold change ≥ 1.3).
5. Heat map depicting dysregulated gene expression (log₂ fold change ≥ 1.3) in T ^Y88A mesodermal cells differentiated in vitro compared to T ^WT from RNA‐Seq of one biological replicate. Gene names correspond to genes that are downregulated and contain a nearby TBS with reduced H3K27ac (fold change > 1.4). Bold gene names indicate known T targets.