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. 2017 Nov 15;19(1):118–134. doi: 10.15252/embr.201744201

Figure 4. H3K27ac is decreased at T binding sites in the TY 88A mutant.

Figure 4

  1. Genome browser tracks depicting H3K27ac/me3 and T binding at the T locus in mesodermal cells differentiated in vitro. Yellow and purple boxes indicate T binding sites with decreased H3K27ac.
  2. ChIP‐qPCR analysis of H3K27ac at the TBS of the T promoter, corresponding to the purple box, in T WT and T Y88A in vitro‐differentiated mesodermal cells at day 4. IgG ChIP was used as a negative control. Error bars depict SD of n = 3–4 biological replicates. *P = 0.005 using a one‐sample t‐test.
  3. Immunoprecipitation of endogenous T from in vitro‐differentiated mesodermal cells at day 4 revealed an interaction with endogenous p300, as assayed by immunoblotting. IgG was used as a negative control. Blot is representative of n = 3 biological replicates.
  4. RT‐qPCR analysis of T expression in KD4‐T mESCs compared to T WT at day 4 of the in vitro differentiation protocol. Doxycycline was administered at day 2 of the protocol to induce expression of shRNA hairpins directed against T 39. The mean of n = 2 biological replicates is depicted.
  5. ChIP‐qPCR analysis of H3K27ac at the T promoter in KD4‐T in vitro‐differentiated mesodermal cells at day 4. The mean of n = 2 biological replicates is depicted.