Table 2.
Gene expression analysis primers
| Primer name | Sequence | Purpose |
|---|---|---|
| T |
CAATGGAGGGGGACAGATCAT CTGGTGATCATGCGTTGCG |
RT–qPCR |
| Lmo2 |
GACCCGTCTGAGGAACCC AGTAGCGGTCCCCTATGTTCT |
RT–qPCR |
| Kdr |
TTCCTTAGGTGCCTCCCCAT CAGGAGCCCGCATTCTAGTT |
RT–qPCR |
| Tal1 |
GTTGTTTACCGCGCAGGAC GAGAGGGGTCGAGGTAGGAC |
RT–qPCR |
| Gata1 |
ACCACTACAACACTCTGGCG CGGTTCACCTGATGGAGCTT |
RT–qPCR |
| Klf1 |
CTTCCTCAAGTGGTGGCGG GTCCTCCGATTTCAGACTCACG |
RT–qPCR |
| Pecam1 |
TCACCAAGAGAACGGAAGGC CTCTTCTCGGGACATGGACG |
RT–qPCR |
| Cdh5 |
GGCAATCAACTGTGCTCTCC GAGGAGCTGATCTTGTCCGT |
RT–qPCR |
| Tie1 |
TACTGTGGCATGACCTGTGC GCATCAGCTCGTACACTTCG |
RT–qPCR |
| Flt1 |
TACCTCACCGTGCAAGGAAC AAGGAGCCAAAAGAGGGTCG |
RT–qPCR |
| Dll4 |
GCAGCTGTAAGGACCAGGA GCAGGTCAAGGTACTATGCTCA |
RT–qPCR |
| Gfi1b |
GCCCTTTGCCTGTGATGTCT CTTCGCTCCTGTGAGTGGAC |
RT–qPCR |
| EpoR |
TAGGCCCCCTCTGTCTCCT GCAGGGCCGCTTTGCTC |
RT–qPCR |
| Hprt |
GCTTTCCCTGGTTAAGCAGTACA GAGAGGTCCTTTTCACCAGCAA |
RT–qPCR |
| Pmm2 |
AGGGAAAGGCCTCACGTTCT AATACCGCTTATCCCATCCTTCA |
RT–qPCR |
| mCherry |
GCCACTACGACGCTGAGG CAACTTGATGTTGACGTTGTAGGC |
RT–qPCR |
| Lmo2 WISH |
AACGAAGCTTAGATACCCCCATCCCTGCTG AACGTCTAGAGTTAGTGCCCAGGAAGAGTCC |
RNA in situ hybridization probe |
| mCherry WISH |
TGGTGAGCAAGGGCGAGGAGG TAATACGACTCACTATAGGGCGGCGCTTCGTACTGTTCCA |
RNA in situ hybridization probe |