Figure 4.

Metal Activation Profile of Arabidopsis GLXI Isoforms.

Absolute activities of GLXI;1 (A), GLXI;2 (B), and GLXI;3 (C) with MGO-GSH and GO-GSH (mmol µg⁻¹ min⁻¹) using different bivalent metal ion cofactors. e, scientific notation (times 10 raised to the power of exponent). Values are means ± se (n = 3) of independent enzyme preparations. Testing for significant differences was performed by two-way ANOVA for GLXI;1 (substrate, P = 0.021; ion, P < 0.0001; interaction, P < 0.0001), for GLXI;2 (substrate, P = 0.20; ion, P < 0.0001; interaction, P < 0.0001), and for GLXI;3 (substrate, P = 0.016; ion, P < 0.0001; interaction, P < 0.0001) (Supplemental File 2), with subsequent Dunnett’s multiple comparisons test to w/oI (without ion) control, a = P < 0.05.