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. 2017 Nov 17;29(12):3234–3254. doi: 10.1105/tpc.17.00258

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Figure 6. — Subcellular Localization of Arabidopsis GLXI Isoforms Using Tobacco Leaf Protoplasts.

(A) The coding sequence of YFP was fused in-frame with the C terminus of the full-length coding sequence of GLXI;1_1, GLXI;2_1, GLXI;3_1, and GLXI;3_4 (GLXI:YFP fusions) and expressed in tobacco leaf protoplasts. GLXI proteins with high probability of mitochondrial localization (AramLocCon Score >3) were coexpressed with the mitochondrial marker protein mito:eq611.

(B) YFP was fused in frame with the N terminus of the full coding sequence of GLXI;1_1, GLXI;2_1, and GLXI;3_4 (YFP:GLXI fusions) and coexpressed with a peroxisomal marker protein in tobacco leaf protoplasts. Images were taken after 3 d of infiltration. merge, protoplast overview; merge_zoom, 50 µm detail; YFP, YFP fluorescence; mitochondria or peroxisome, fluorescence of marker proteins for the organelles; Chl_auto, autofluorescence of chlorophyll; n.r., not required. Bar = 10 µm.