Effect of Sucrose, MGO, and GO on the Germination and Seedling Establishment of Arabidopsis GLXI T-DNA Insertion Lines.
(A) Germination (in percentage of total seeds sown) at 2 d after seed imbibition. The emergence of the radicle was used as an indicator of germination. Values are means ± se (n = 4) from independent experimental trials with 50 to 100 counted seeds for each genotype. Testing for significant differences was performed by two-way ANOVA on arcsine transformed data (treatment, P < 0.0001; genotype, P < 0.0001; interaction, P = 0.0005).
(B) Root length after 12 d of germination, values are means ± se (n = 4) from independent experimental trials with 7 to 10 root measurements each. Testing for significant differences was performed by two-way ANOVA (treatment, P < 0.0001; genotype, P < 0.0001; interaction, P < 0.0001) (Supplemental File 2). Tukey’s multiple comparison test was used, where “a” indicates significant differences in treatments versus MS control and “b” indicates significant differences in genotypes versus Col-0 control.
(C) Photographs of Col-0 and glxI;3 loss of function lines after 12 d of germination in MS medium and in the presence of sucrose, MGO, and GO. Bar = 1 cm.