Fig. 4.

Silencing AFAP1-AS1 inhibited cell migration and invasion in TSCC cells. a Cell invasion was measured in SCC-9 and CAL-27 cell lines transfected with shR-AFAP1-AS1 or shR-NC through transwell assays with Matrigel. b Cell migration was measured in SCC-9 and CAL-27 cell lines transfected with shR-AFAP1-AS1 or shR-NC through transwell assays without Matrigel. c Cell migration was analyzed by a wound healing assay. SCC-9 and CAL-27 cells were seeded in 6-well plates and grown to full confluence. Cells were treated with shR-AFAP1-AS1 or shR-NC. After 48 h, the wounding space was observed and the cells were photographed. The data represent the mean ± SDs of 3 replicates. *Indicates a significant difference between the Mock group and shR-AFAP1-AS1 group, #indicates a significant difference between the shR-NC group and the shR-AFAP1-AS1 group. * P < 0.05; ** P < 0.01; *** P < 0.001; # P < 0.05; ## P < 0.01;### P < 0.001