Fig. 6.

Downregulation of AFAP1-AS1 suppressed the expression of EMT-related genes and tumor growth in vivo. a Nude mice were transplanted subcutaneously with CAL-27 cells transfected with AFAP1-AS1 shRNA or the control shRNA. After 35 days, the mice were sacrificed. A representative picture of the morphology of the nude mice after sacrifice.b A representative picture of the morphology of tumor xenografts after excision at 35 days of treatment.c Tumor volumes were measured weekly from week one to five post-injection.d Tumor weights were measured after the mice were sacrificed. e The expression of AFAP1-AS1 in xenograft tumors was detected by qRT-PCR. f The effect of AFAP1-AS1 on the expression of EMT-related genes in xenograft tumors was analyzed by qRT-PCR. The data represent the mean ± SD of 3 replicates. *Indicates a significant difference between the Mock group and shR-AFAP1-AS1 group, #indicates a significant difference between the shR-NC group and the shR-AFAP1-AS1 group. * P < 0.05; ** P < 0.01; *** P < 0.001; # P < 0.05; ## P < 0.01;### P < 0.001