| Antibodies |
| Rabbit anti-β-Gal |
MP |
559761 |
| Mouse anti-β-Gal |
Promega |
Z378A |
| Rabbit anti-GFP |
ThermoFisher |
A6455 |
| Mouse anti-Wg |
DSHB |
4D4 |
| Mouse anti-En |
DSHB |
4D9 |
| Mouse anti-Scr |
DSHB |
6H4.1 |
| Mouse anti-Ubx |
DSHB |
FP3.38 |
| Mouse anti-Antp |
DSHB |
4C3 |
| Rabbit anti-Abd-A |
Santa Cruz |
d-130 |
| Guinea Pig-anti-Sna |
gift from Yutaka Nibu, Cornell University, USA |
|
| Rabbit anti-Vg |
gift of Sean Carroll University of Wisconsin-Madison, USA |
|
| Guinea Pig anti-Dll |
[19] |
|
| Guinea Pig anti-Hth |
[19] |
|
| Rabbit anti-Doc-2 |
gift from Ingof Reim, Friedrich-Alexander University Erlangen-Nürnberg, Germany |
|
| Rabbit anti-Phospho-p44/42 MAPK |
Cell Signaling |
9101 |
| Experimental Models: Organisms/Strains |
|
|
|
prd-Gal4
|
Flybase |
FBtp0000358 |
| esgNP5130-Gal4 |
Flybase |
FBal0098823 |
| DllMD23-Gal4 |
Flybase |
FBti0002783 |
|
Doc-1-Gal4 (GMR 45H05) |
Flybase |
FBsf0000164776 |
| Dll304-Gal4 |
Flybase |
FBal0288749 |
|
esg05730-lacZ
|
Flybase |
FBti0008070 |
| dpp10638 -lacZ |
Flybase |
FBti0002737 |
|
act5C>stop>lacZ; UAS-flp
|
[46] |
|
| Scr4 |
Flybase |
FBal0015280 |
| Antp25 |
Flybase |
FBal0000566 |
| Ubx1 |
Flybase |
FBal0017338 |
| UbxMx12abd-AM1 |
Gift from Ernesto Sanchez-Herrero |
|
| Df(btd,Sp1) |
Flybase |
FBab0047246 |
| EGFRnull |
Flybase |
FBal0066102 |
| Df(3l)DocA |
Flybase |
FBab0037663 |
| tkva12 |
Flybase |
FBal0016821 |
| vgnull |
Flybase |
FBal0093753 |
| snaV2 |
Flybase |
FBal0015896 |
| UAS-rpr
|
Flybase |
FBst0005823 |
| UAS-hid
|
Flybase |
FBtp0012437 |
| UAS-GFP
|
Flybase |
FBti0012493 |
| UAS-TCFDN
|
Flybase |
FBtp0001721 |
| UAS-arm* (delta N) |
Flybase |
FBtp0001725 |
| UAS-brk
|
Flybase |
FBtp0085350 |
| UAS-tkvQD
|
Flybase |
FBtp0001199 |
| UAS-Doc-2
|
Flybase |
FBtp0017741 |
| UAS-EGFRλtop4.2
|
Flybase |
FBtp0008722 |
| UAS-Scr
|
Flybase |
FBtp0000719 |
| UAS-Antp
|
Flybase |
FBtp0014554 |
| UAS-Ubx
|
Flybase |
FBal0039098 |
| UAS-abd-A
|
Flybase |
FBtp0085557 |
| UAS-Abd-B
|
Flybase |
FBal0038086 |
| UAS-vg
|
Flybase |
FBtp0051400 |
| UAS-sna
|
Flybase |
FBtp0009053 |
| Recombinant Proteins |
| His-tag HM (Hth) |
[40] |
|
| His-tag Exd |
[40] |
|
| His-tag Dfd |
[40] |
|
| His-tag Antp |
[40] |
|
| His-tag Ubx |
[40] |
|
| His-tag Abd-A |
[40] |
|
| Oligonucleotides |
| sna-DP sense: cagtAAGCTTgtggagcgcaccccaaagct |
|
|
| sna-DP asense: cagtAGATCTaagggatctgataaagaacgatctcc |
|
|
| sna-1.7 sense: cagtAAGCTTggttggggttaaagtagagcggc |
|
|
| sna-1.7 asense: cagtAGATCTtggcaaccgactaacaacgcatc |
|
|
| sna-1.7-1 sense: cagtAAGCTTggttggggttaaagtagagc |
|
|
| sna-1.7-1 asense: cagtAGATCTttgatcttgcgggtaagccc |
|
|
| sna-1.7-2 sense: cagtAAGCTTttcttatgggcttacccgca |
|
|
| sna-1.7-2 asense: cagtAGATCTcaaagctcagcagcggcagc |
|
|
| sna-1.7-3 sense: cagtAAGCTTgctgccgctgctgagctttg |
|
|
| sna-1.7-3 asense: cagtAGATCTatacgttaggcattgctatc |
|
|
| sna-1.7-4 sense: cagtAAGCTTtagcaatgcctaacgtatcg |
|
|
| sna-1.7-4 asense: cagtAGATCTtggcaaccgactaacaacgc |
|
|
| sna-1.7-2A sense: cagtAAGCTTttcttatgggcttacccgca |
|
|
| sna-1.7-2A asense: cagtAGATCTggtaggaataaaccggaggag |
|
|
| sna-1.7-2B sense: cagtAAGCTTgaatggcgcccgcctcgattt |
|
|
| sna-1.7-2B asense: cagtAGATCTcaaagctcagcagcggcagc |
|
|
| sna-1.7 sense pEntry: CACCggttggggttaaagtagagcggc |
|
|
| sna-1.7-2 sense pEntry: CACCttcttatgggcttacccgca |
|
|
| sna-1.7-3 sense pEntry: CACCgctgccgctgctgagctttg |
|
|
| Mad-1 sense: gtccgccattaaacgatATCATAAtgtTAATtatgtttacagatttgtcg |
|
|
| Mad-1 asense: cgacaaatctgtaaacataATTAacaTTATGATatcgtttaatggcggac |
|
|
| Mad-2 sense: ccggtttattcctaccgaatTTATAAATTTtcgattttattaccttc |
|
|
| Mad-2 asense: gaaggtaataaaatcgaAAATTTATAAattcggtaggaataaaccgg |
|
|
| Mad-3 sense: ccttatctatcggaccggtctTAAGTAAATAAtgtctgtctgtccccatatctttcagg |
|
|
| Mad-3 asense: cctgaaagatatggggacagacagacaTTATTTACTTAagaccggtccgatagataagg |
|
|
| Hox-1 Δ2 sense: agatttacgacagcatttcaCCCCttatgtcacattctaggg |
|
|
| Hox-1 Δ2 asense: ccctagaatgtgacataaGGGGtgaaatgctgtcgtaaatct |
|
|
| Hox-2 sense: gcgcatctccgccgtaaaccGTCGTCACGttttatgttaatgcaac |
|
|
| Hox-2 asense: gttgcattaacataaaaCGTGACGACggtttacggcggagatgcgc |
|
|
| Hox-3 sense: tgatctgcgatcgacccaagCGGGCATCAagcatttcataatttatgtc |
|
|
| Hox-3 asense: gacataaattatgaaatgctTGATGCCCGcttgggtcgatcgcagatca |
|
|
| Hox-4 sense: ggctaagcgcatctccTACTGCCCAAGGatgacatttttatgttaatgc |
|
|
| Hox-4 asense: gcattaacataaaaatgtcatCCTTGGGCAGTAggagatgcgcttagcc |
|
|
| Hox-5 sense: gggccatttaattgtctcgaCGTCCGGTAGTAcgctgctgagctttg |
|
|
| Hox-5 asense: caaagctcagcagcgTACTACCGGACGtcgagacaattaaatggccc |
|
|
| Hox-6 sense: cgaaccatttgaaaatacccccgccCGCCGGCGTTCgccccgctcctattcagttgcaaa |
|
|
| Hox-6 asense: tttgcaactgaataggagcggggcGAACGCCGGCGggcgggggtattttcaaatggttcg |
|
|
| Recombinant DNA |
| attB-hs43-nuc-lacZ |
[19] |
|
| pBPGUw (Gal4) |
[47] |
|
| Software and Algorithms |
|
|
| Image J |
https://imagej.nih.gov/ij/ |
|
| JASPAR |
http://jaspar.genereg.net/) |
|
| Target Explorer |
http://te.cryst.bbk.ac.uk/ [48] |
|
| Vienna Drosophila Resource Center |
http://enhancers.starklab.org/ |
|
| Other |
